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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/11/1999 |
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Data da última atualização: |
16/11/1999 |
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Autoria: |
MELLONI, R.; CARDOSO, E. J. B. N. |
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Afiliação: |
FAPESP; ESALQ/USP; CNPq. |
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Título: |
Quantificacao de micelio extrarradicular de fungos micorrizicos arbusculares em plantas citricas e endofitos. Metodos empregado |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Revista Brasileira de Ciencia do Solo, v.23, n.1, p.53-58, Vicosa, 1999 |
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ISSN: |
0100-0683 |
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Idioma: |
Português |
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Conteúdo: |
Otimizacao de metodos de extracao e avaliacao de micelio extrarradicular ativo (MEA) e total (MET) constituem os principais passos para um estudo mais profundo da interacao fungo micorrizico arbuscular (FMA) e hospedeiro, ja que a absorcao e translocacao dos nutrientes do substrato ao hospedeiro sao realizadas, respectivamente, por tais estruturas fungicas. Metodos de extracao de micelio extrarradicular de FMAs propostos por diversos autores foram reavaliados e mesclados, originando um metodo exclusivo e de facil execucao, juntamente com a calibracao de metodos para avaliacao e quantificacao de MEA (fluorescencia induzida com a hidrolise do diacetato de fluoresceina e reducao de iodonitrotetrazolio), em substrato arenoso. |
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Palavras-Chave: |
FDA; FDA hydrolysis; INT; Reduction stain. |
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Thesagro: |
Corante. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01341naa a2200205 a 4500
001 1635629
005 1999-11-16
008 1999 bl uuuu u00u1 u #d
022 $a0100-0683
100 1 $aMELLONI, R.
245 $aQuantificacao de micelio extrarradicular de fungos micorrizicos arbusculares em plantas citricas e endofitos. Metodos empregado
260 $c1999
520 $aOtimizacao de metodos de extracao e avaliacao de micelio extrarradicular ativo (MEA) e total (MET) constituem os principais passos para um estudo mais profundo da interacao fungo micorrizico arbuscular (FMA) e hospedeiro, ja que a absorcao e translocacao dos nutrientes do substrato ao hospedeiro sao realizadas, respectivamente, por tais estruturas fungicas. Metodos de extracao de micelio extrarradicular de FMAs propostos por diversos autores foram reavaliados e mesclados, originando um metodo exclusivo e de facil execucao, juntamente com a calibracao de metodos para avaliacao e quantificacao de MEA (fluorescencia induzida com a hidrolise do diacetato de fluoresceina e reducao de iodonitrotetrazolio), em substrato arenoso.
650 $aCorante
653 $aFDA
653 $aFDA hydrolysis
653 $aINT
653 $aReduction stain
700 1 $aCARDOSO, E. J. B. N.
773 $tRevista Brasileira de Ciencia do Solo$gv.23, n.1, p.53-58, Vicosa, 1999
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá; Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
31/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ANDRADE-PORTO, S. M.; RAMOS, C. A.; ROQUE, R.; AFFONSO, E. G.; BARCELLOS, J. F. M.; QUEIROZ, M. N.; ARAÚJO, C. S. O.; TAVARES-DIAS, M. |
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Afiliação: |
SANNY M. ANDRADE-PORTO, UFAM; CLEVERSON A. RAMOS, UFAM; ROSEMARY ROQUE, INPA; ELIZABETH G. AFFONSO, INPA; JOSÉ F. M. BARCELLOS, UFAM; MARIETA N. QUEIROZ, Universidade Nilton Lins; CLEUSA S. O. ARAÚJO, UEA; MARCOS TAVARES DIAS, CPAF-AP. |
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Título: |
Histopathological evaluation of formalin toxicity in Arapaima gigas (Arapaimidae), the giant fish from Amazon. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 38, n. 6, p. 1015-1025, Jun. 2018 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to determine the lethal concentration and the structural and ultra-structural effects caused by the formalin exposure on juveniles of Arapaima gigas. Ninety fish (60.1± 2.5g and 20.2±0.9cm) were exposed to 0, 22, 44, 66, 88 and 110mg L-1 in order to determine the lethal concentration (LC50-96h) that was 36.4mg L-1 of formalin. Sublethal effects were evaluated using histopathological analysis on the gills and assessment of behavioral alterations and clinical signs. The LC50 of formalin for 24, 48 and 72h was 88.3, 64.7 and 56.8mg L-1 respectively. Clinical signs and behavioral changes were found: erratic swimming, lethargy, crowding on the water surface, loss of hydrodynamic equilibrium, spasms and agonistic confrontation, which were observed only at 88 and 110mg L-1. The histological alteration index (HAI) showed that 66, 88 and 100mg L-1 presented significant difference (p<0.05) in relation to unexposed fish, indicating that moderate damage to the gills of fish exposed to formalin had occurred. The mean values of alteration (MVA) for 22, 44, 66, 88 and 110mg L-1 were 1.14, 1.29, 1.51, 1.53 and 1.60 respectively, and differences in this index were only observed with 110 mgL-1 of formalin. It is therefore possible to conclude that sublethal concentrations of formalin (22.0mg L-1) did not compromise the health of juveniles of A. gigas. Finally, concentrations greater than to LC50-96h may be carefully used for short-term exposure, since the MVA for all concentrations tested only indicated localized lesions that did not compromise gills functionality of exposed fish. MenosThis study aimed to determine the lethal concentration and the structural and ultra-structural effects caused by the formalin exposure on juveniles of Arapaima gigas. Ninety fish (60.1± 2.5g and 20.2±0.9cm) were exposed to 0, 22, 44, 66, 88 and 110mg L-1 in order to determine the lethal concentration (LC50-96h) that was 36.4mg L-1 of formalin. Sublethal effects were evaluated using histopathological analysis on the gills and assessment of behavioral alterations and clinical signs. The LC50 of formalin for 24, 48 and 72h was 88.3, 64.7 and 56.8mg L-1 respectively. Clinical signs and behavioral changes were found: erratic swimming, lethargy, crowding on the water surface, loss of hydrodynamic equilibrium, spasms and agonistic confrontation, which were observed only at 88 and 110mg L-1. The histological alteration index (HAI) showed that 66, 88 and 100mg L-1 presented significant difference (p<0.05) in relation to unexposed fish, indicating that moderate damage to the gills of fish exposed to formalin had occurred. The mean values of alteration (MVA) for 22, 44, 66, 88 and 110mg L-1 were 1.14, 1.29, 1.51, 1.53 and 1.60 respectively, and differences in this index were only observed with 110 mgL-1 of formalin. It is therefore possible to conclude that sublethal concentrations of formalin (22.0mg L-1) did not compromise the health of juveniles of A. gigas. Finally, concentrations greater than to LC50-96h may be carefully used for short-term exposure, since the MVA for all concentr... Mostrar Tudo |
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Palavras-Chave: |
Formalina; Toxicidade; Toxicoses; Treatment. |
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Thesagro: |
Histopatologia; Peixe de Água Doce; Pirarucu; Tratamento. |
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Thesaurus NAL: |
Arapaima gigas; Arapaimidae; Formalin; Freshwater fish; Histopathology; Toxicity. |
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Categoria do assunto: |
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S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183018/1/CPAF-AP-2018-Histopathological-evalution-of-formalin.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182143/1/Histopathological-evaluation-of-formalin-toxicity.pdf
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Marc: |
LEADER 02660naa a2200373 a 4500
001 2095738
005 2018-10-31
008 2018 bl uuuu u00u1 u #d
100 1 $aANDRADE-PORTO, S. M.
245 $aHistopathological evaluation of formalin toxicity in Arapaima gigas (Arapaimidae), the giant fish from Amazon.$h[electronic resource]
260 $c2018
520 $aThis study aimed to determine the lethal concentration and the structural and ultra-structural effects caused by the formalin exposure on juveniles of Arapaima gigas. Ninety fish (60.1± 2.5g and 20.2±0.9cm) were exposed to 0, 22, 44, 66, 88 and 110mg L-1 in order to determine the lethal concentration (LC50-96h) that was 36.4mg L-1 of formalin. Sublethal effects were evaluated using histopathological analysis on the gills and assessment of behavioral alterations and clinical signs. The LC50 of formalin for 24, 48 and 72h was 88.3, 64.7 and 56.8mg L-1 respectively. Clinical signs and behavioral changes were found: erratic swimming, lethargy, crowding on the water surface, loss of hydrodynamic equilibrium, spasms and agonistic confrontation, which were observed only at 88 and 110mg L-1. The histological alteration index (HAI) showed that 66, 88 and 100mg L-1 presented significant difference (p<0.05) in relation to unexposed fish, indicating that moderate damage to the gills of fish exposed to formalin had occurred. The mean values of alteration (MVA) for 22, 44, 66, 88 and 110mg L-1 were 1.14, 1.29, 1.51, 1.53 and 1.60 respectively, and differences in this index were only observed with 110 mgL-1 of formalin. It is therefore possible to conclude that sublethal concentrations of formalin (22.0mg L-1) did not compromise the health of juveniles of A. gigas. Finally, concentrations greater than to LC50-96h may be carefully used for short-term exposure, since the MVA for all concentrations tested only indicated localized lesions that did not compromise gills functionality of exposed fish.
650 $aArapaima gigas
650 $aArapaimidae
650 $aFormalin
650 $aFreshwater fish
650 $aHistopathology
650 $aToxicity
650 $aHistopatologia
650 $aPeixe de Água Doce
650 $aPirarucu
650 $aTratamento
653 $aFormalina
653 $aToxicidade
653 $aToxicoses
653 $aTreatment
700 1 $aRAMOS, C. A.
700 1 $aROQUE, R.
700 1 $aAFFONSO, E. G.
700 1 $aBARCELLOS, J. F. M.
700 1 $aQUEIROZ, M. N.
700 1 $aARAÚJO, C. S. O.
700 1 $aTAVARES-DIAS, M.
773 $tPesquisa Veterinária Brasileira$gv. 38, n. 6, p. 1015-1025, Jun. 2018
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Embrapa Amapá (CPAF-AP) |
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